ࡱ> ceb'` RVPbjbj2Zrr8& 4Z 2 """1111111$3h5X29"""""2 4U2..."B   1."1... `,P./k202.M6.:M6..M6/T""."""""22."""2""""   ~ހ;m'`Km[el ~ހ;m'`Km[el gSv݄grl0KQƖ= b_bl03H>e\'` TMO }ceQl0 MTTlI{0vQ-NMTTlNvQ_{O NyrkhKmNhV0e>e\'` TMO }0T'YybϑhKmvyrp _0R^lv^(u0FOMTTlb_bvFormazan :N4l Nn'`v R g:gnBRn 1uN(WS Nnd\OeO gS&^p\RvFormazan Ee ge͑ Y'`eu]0:NN㉳Qُ*N xvzNXTS_SN_ Yy4ln'`vV.lUv{|YXTT0CCK-8WST-8 I{0 s1\ُ NyV.lUv{|el\ON*N{USN~ 1.MTTl MTTSf[ T3-(4 5-N2uW{VU-2)-2 5-NWV.lUnv FUT T{VU݄0hKmSt:N;m~ހ~|SO-Nv%tsx1 "lvOYn'`MTT؏S:N4l Nn'`v݄+}r~vf2u܁Formazan v^ly(W~ހ-N {k~ހedkR0N2uWNxDMSO n~ހ-Nv 2u (uvTMQuhKmN(W490nmlYKm[vQIQ8T6e

e\Oea'`Km[I{0[vyrp/fupOe^ؚ0~Nm0 :p1uNMTT~؏S@bNuv2uNir NnN4l nTMbhKm0ُ NNO]\OϑXR _NO[[~gvQnx'`Nuq_T Nn2uv g:gnBR[ [_N g_c[0 2.XTTl XTTSf[ T2,3-bis(2-methoxy-4-nitro-5- sulfophenyl)-5-[(phenylamino)carbonyl] -2H-tetrazoliumhydroxide \O:N~|SO1"lvv\O(u^ir ;m~ހ؏Sb4ln'`vYjĞr2uNir0S_XTTN5uP[vPTBROY PMS TT^(ue vQ@bNuv4ln'`v2uNirv8TIQ^N;m~ހvpeϑbckk0 Op10O(ueO wSNmm~ހ20hKm_30upOe^ؚ uSNKm[NO~ހ[^40͑ Y'`ONMTT0 :pXTT4lnm N3z[ NO)nOX[bsMs(u0 3.CCK-8lbyWST-8l CCK-8ՋBR-N+T gWST 8Sf[ T2-(2-2u'lW-4-]xWW)-3-(4-]xWW)-5-(2,4-Nxx)-2H-VUUSv] [ (W5uP[}SO1-2u'lW-5-2uW)TUkxxN2uo1-MethoxyPMS v\O(u N~ހ~|SO-Nv1"lv؏S:NwQ gؚ^4ln'`vĞr2u܁Nir Formazan 0ubv2uirvpeϑN;m~ހvpeϑbckk0(uvTMQuhKmN(W450nmlYKm[vQIQ8T6e

e\'` TMO }T g:gnBR20hKm_30upOe^ؚ uSNKm[NO~ހ[^40͑ Y'`ON MTT50[~ހk'`\60:N1tnm k6R sS_sS(u0 :p:10NMTTvk CCK-8TXTTvNe\Oea'`Km[I{0[vyrp/fupOe^ؚ0~Nm0 00:p1uNMTT~؏S@bNuv2u܁Nir NnN4l nTMbhKm0ُ NNO]\OϑXR _NO[[~gvQnx'`Nuq_T Nn2uv g:gnBR[[_N g_c[0 MTT nmvM6Rel 8^ dkl-NvMTTSm^:N5mg/ml0Vdk SNySMTT 0.5KQ nN100 mlvxxQmPBS beZ~vW{QW-N (u0.22mnǏn NdSnm̑v~̃ >e4! IQOX[sSS0(WM6RTOX[vǏ z-N [hVg}Y(uݔ{~SOO0[vePbN,sQ핅QS NveIQopegIQ ɉ_ُ7hk}Y0 lav/f MTTlS(ueghKm~ހv[peTv[;mR FO NKm[~ހ~[pe0(W(uvhNhKm~ "$ΧΜΜΧΜΜ΁΁΁vΧ΁΁΁΁v΁΁΁hxhxKHaJhxh<KH^Jhxhx5KH\^JaJhxhxKH^JhO!KH^JaJo(hO!hx5KH\^Jhxhx5KH\^JhxhxKH^JaJ&hO!hx0J5CJ OJQJaJ o(hO!hx5CJ OJQJaJ . $dd$1$If[$\$a$gdO! $d1$[$a$gdx$d1$[$`a$gdx$gd00[$\$^g`a$gdx "PTPQ<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!T Q<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!T "&(,.68@BDLN\^fhprt  "$&(*46<>BDFHJTV\^hxhxKH^JaJhxhxKHaJhxh<KH^JW "(.8BQ<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!TBDN^hrQ<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!TrtQ<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!TQ<<<<$dd$1$If[$\$a$gdxkdj$$IfTx\y [GeR 0634xabytO!TQ<<<<$dd$1$If[$\$a$gdxkdL$$IfTx\y [GeR 0634xabytO!TQ<<<<$dd$1$If[$\$a$gdxkd.$$IfTx\y [GeR 0634xabytO!T Q<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!T $(Q<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!T(*6>DHQ<<<<$dd$1$If[$\$a$gdxkd$$IfTx\y [GeR 0634xabytO!THJV^bfQ<<<<$dd$1$If[$\$a$gdxkd $$IfTx\y [GeR 0634xabytO!T^`bdfhl$,.DNP@|@4@>@F@H@h@˺yjWyUyU$hxhxB*CJOJQJo(phhO!hxCJOJQJ^JhjmCJOJQJo(hO!CJOJQJo(hxhxCJOJQJo(hxhxCJOJQJhxhx0JCJOJQJo( hxhx0JCJOJQJaJ #hxhx0JCJOJQJaJ o(hxhxKHaJhxh<KH^JhxhxKH^JaJ!fhjlQ::::gd00[$\$^g`gdxkd $$IfTx\y [GeR 0634xabytO!T.H@vA:BBVEIJKLMNbNTO Pd[$\$gd<d[$\$gdxd00[$\$gd<d[$\$gdjmd00[$\$gdjmd00[$\$gdxd[$\$`gdxgveP :NNO[~gv~'` MTT8TIQ^g}Y(W0-0.7VQ0 MTTN,g}Ys(usM ǏnT4!IQOX[$NhTQ gHe bM6Rb5mg/mlOX[(W-20^gOX[ MQS YQ g}Y\BRϑRň (uIQb/fў~0!{~SOOIQNMQR0bN,bMTT|Rň(WEP{̑ (uvePsM vc_W{Qg-NR l_N NP[MHNY $\vQS_MTTS:Npp~re1\~[ NQ(uN0 MTT gLv'` (uveP\_ gagNg}Y&^yfv?|KbWY0MbvMTTẽ MTT[̃_Oea_96T[gRe NIQ_Nl gsQ| kzew b`O N>e_vePSNbd\OS NvgqfopsQc0 M6RMTTe(uPBSph=7.4 n0PBSMeNaCl 8g KCl 0.2g Na2HPO4 1.44g KH2PO4 0.24gpH 7.4 [[1L0 nfMTTl[ek 1cy~ހ(u+T10 ΀\[r@n_W{QmMbUS*N~ހ`m NkT[1000-10000*N~ހcy0R96T[g kT[SOy200ul0 2W{Q~ހ TN,W{QagN W{Q3-5)YS9hncՋvvTBlQ[W{Qe 0 3HTrW{Q3-5)YT kT[RMTTnm5mg/ml(uPBSM6R pH=7.4)20ul0~~u[4 h ~bkW{Q \_8T_T[QW{Q Nnm [N`nm~ހy_TQ8T_T[QW{Q Nnm0kT[R150ul DMSO /ca10min O~vfirEQRn0 4kr b490nml (WvTMQuvKmN NKm[TT[IQ8T6e

f_\ N‰[0 4kT[ReQ20ulMTTnm5mg/ml sS0.5%MTT ~~W{Q4h0oirNMTTYS^ SHQy_T_SW{Qm \_(uPBSQ2-3MT QReQ+TMTTvW{Qm0 5~bkW{Q \_8TST[QW{Qm0 6kT[ReQ150ulN2uWNx nGd^ NNO/ca10min O~vfirEQRn0(WvTMQuhKmNOD490nmY KmϑTT[v8TIQf_\ N‰[0 3kT[ReQ10 ul MTTnm5 mg/ml sS0.5%MTT ~~W{Q4 h0`nm~ހcPO(uWST-1 W{Q4 hTSǏek4 vcvTMQuhKmNOD570nm630nm!hQ KmϑTT[v8TIQ

P@PBPDPFPHPJPLPNPPPTPVPhb,h}jh}U hxh2'BhxhxCJOJQJ$hxhxB*CJOJQJo(ph P"P&P(P,P.P2P4P8P:PP@PBPDPFPHPJPLPNPPPRPTPVP &dPgdb,gdx0182P. A!"#$%S $$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!T$$If!vh55R55 #v#vR#v#v :V x0655R55 / 34xytO!TN@N cke $1$a$$CJKHPJ_HaJmH nHsH tH\@\ xh 1$dd1$@&[$\$a$5CJ0KH$OJQJ\^JaJ0\@"\ xh 2$dd1$@&[$\$a$5CJ$KHOJQJ\^JaJ$J@J xh 3$$d@&5CJ \aJ $A@$ ؞k=W[SOBi@B nfh*phO xtdep clearfix nbw-act fc06$dd1$[$\$a$CJKHOJQJ^J"O!" xpleftO1 xsep<OA< xpright fc07 ztag.OQ. x ul sep fc042Oa2 x ul sep fc04bOqb x%sep ul fc04 selected js-fcurrent fc05"W@" xp5\VOV xwestern$dd1$[$\$a$CJKHOJQJ^JN@N b,u w'$ 9r &dG$Pa$CJaJ< @< b,u$ 9r G$a$CJaJZ ]tAIJOTcdkoswx} "$%+/13456KR  j  I8|$    000000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0000000000000000000000ȑ00ȑ00ȑ00ȑ00q@00q@0ȑ0]tAIJOTcdkoswx} "$%+/134KR  j  I8|$00]00]00]00]00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 0 0]0 0]0 0]0 0]0 0 0]0 0]0 0]0 0]0 00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 00]00]00]00]0 00 00 00 00 0 @0@0@0@0@0@0@0@0@0@0@0@0@0@0@0@0@0@0 0  ^h@TFOVP )*+ Br (Hf PVP,TP@  @ 0(  B S  ?H0(   #%/38;ilpxy  =>EHWX`}  LMST\hlqt !$%34:;NOUXYZ]`s$%=B]^ =>@AHMNRSY[`bsvx| %*PQUX`qPoJNT[_ju &   " % ( + / 3 4 5 8 > ? N O \ ] j l o u w    L O v w | }   9 B   #&(=NOUW\hrtx{ +79F #()+25:;@DGHJMNRS}~ \]st@AHJNOSTbdjknorsvx| %*6JKQR    i j ~  HI78{|#$ATd6< )^|gtfi4X9,!+Jb[^$_ecdX9)^|gSs$}O!:&2'B"4V^b/sjmb,<_x-IJOTcdkoswx} "$%+/134@Ta 4PPPP@UnknownGz Times New Roman5Symbol3& z Arial;[SOSimSun qhzz!-!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[r2HX ?x2~ހ;m'`Km[el Cyclin ChouUserOh+'0|  8 D P\dltϸԲⶨ Cyclin ChouNormalUser2Microsoft Office Word@F#@@z՜.+,0 X`lt| '   !"#$%&'()*+,-/012345789:;<=>?@ABCDEFGHIJKLMNOPQSTUVWXY[\]^_`adRoot Entry Fp~!fData .1Table6M6WordDocument2ZSummaryInformation(RDocumentSummaryInformation8ZCompObjm  FMicrosoft Office Word ĵ MSWordDocWord.Document.89q